First Report of Potato mop top virus Causing Potato Tuber Necrosis in Colorado and New Mexico
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چکیده
منابع مشابه
Evolutionary features of 8K (KDa) silencing suppressor protein of Potato mop-top virus
The cysteine-rich 8K protein of Potato mop-top virus (PMTV) suppresses host RNA silencing. In this study, evolutionary analysisof 8K sequences of PMTV isolates was studied on the basis of nucleotide and amino acid sequences. Twenty-one positively selected sites were identified in 8K codingregions. Recombination events were found in the 8K of PMTV isolates with a rate of 1.8. Totally 30 haplotyp...
متن کاملDetection of potato mop-top virus capsid readthrough protein in virus particles.
Potato mop-top furovirus (PMTV) RNA 3 encodes the 20 kDa coat protein and a larger readthrough protein of 67 kDa. The readthrough protein is expressed by suppression of the amber stop codon which terminates the coat protein gene. A 21 kDa C-terminal fragment of the readthrough protein was doned, fused to glutathione S-transferase and expressed in E. coli. An antiserum prepared against purified ...
متن کاملThe Role of Microtubule Association in Plasmodesmal Targeting of Potato mop-top virus Movement Protein TGBp1
Cell-to-cell movement of Potato mop-top virus (PMTV) is mediated by three virus-encoded 'triple gene block' (TGB) proteins termed TGBp1, TGBp2 and TGBp3. TGBp1 binds virus RNAs to form viral ribonucleoprotein complexes (vRNPs), the transport form of viral genome. TGBp2 and TGBp3 are necessary for intracellular delivery of TGBp1-containing vRNPs to plasmodesmata. To analyze subcellular localizat...
متن کاملCell-to-Cell Movement and Protein Interactions of Potato mop top virus
27 28 Functions of viral proteins can be regulated through phosphorylation by serine/threonine kinases 29 in plants but little is known about involvement of tyrosine kinases in plant virus infection. In this 30 study, TGBp3, one of the three movement proteins encoded by a triple gene block (TGB) of 31 Potato mop-top virus (PMTV), was detected for the first time in PMTV-infected plants and 32 fo...
متن کاملDigoxigenin-labelled molecular probe for the simultaneous detection of three potato pathogens: potato spindle tuber viroid (PSTVd), potato virus Y (PVY), and potato leafroll virus (PLRV).
A molecular probe, p3POT, was constructed of PSTVd, PVY, PLRV cDNA fragments introduced into pUC18 vector. Sequencing of the inserts revealed that cloned fragments covered conservative parts of pathogenic genomes. Dot-blot hybridization of digoxigenin-labelled construct to crude extracts from plants infected with different potato viruses proved high sensitivity and specificity of the p3POT prob...
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ژورنال
عنوان ژورنال: Plant Disease
سال: 2015
ISSN: 0191-2917,1943-7692
DOI: 10.1094/pdis-08-14-0819-pdn